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KMID : 0613819970070030172
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Journal of Life Science
1997 Volume.7 No. 3 p.172 ~ p.175
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Effect of ozone treatment on the expression of a foreign gene in transgenic poplar subclones
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Abstract
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Transgenic hybrid poplar subclones containing herbicide glyphosate resistant gene (aroA) were treated with ozone at the concentration of 100 nL L$^{-1}$ for 6 hr for 5 consecutive days. The foreign gene expression in leaves of all treated plants was reduced both at transcriptional and translational levels confirmed by Northern and Western blot analysis, respectively, as compared to non-treated control plants. These results indicated that the expression of foreign gene in transgenic plants could be affected by the environmental stresses. Thus, the performance of transgenic plants cultivated on field conditions may be lower than they are expected.
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